Abstract The development of additive manufacturing (AM) technology provides higher feasibility for designing and manufacturing lattice structures. However, the manufacturing process usually generates residual deformation and stress, and even produces cracking, thus affecting the performance of the parts. This work establishes a simulation model of the Ti-6Al-4V lattice structures during laser powder bed fusion (LPBF) based on the inherent strain method. Effects of geometric lattice parameters (inclination angle, rod diameter, rod length) on the residual deformation and stress are analyzed. Based on the simulation results, measures for improving the quality of the lattice structures are proposed. The proposed model and simulation results can provide theoretical references for designing and manufacturing the lattice structures during practical engineering applications of LPBF. AM (AM However cracking parts Ti6Al4V TiAlV Ti 6Al 4V Al V LPBF (LPBF method inclination angle diameter length analyzed

Abstract To investigate effects of high-fat diet (HFD) on body length, weight and depression-like behavior of prenatal stress (PS) offspring rats. PS rat model was established by restraint stress. Sucrose preference and forced swimming tests were performed. Moreover, the effects of HFD on the growth and depression-like behavior in offspring rats were observed. Length of male and female HF group was significantly longer than that control, but length of male PS+HF group was significantly shorter than HF group. Body weight of male and female HF group was significantly higher than control, and body weight of male PS+HF group was significantly lower than HF group. No significant differences were observed in length between female PS +HF and HF groups. Compared with male PS+HF group, growth rate of male HF group was significantly higher. Moreover, HFD could reduce sucrose preference, and prolong immobility time of PS offspring rats. HFD can promote weight gain of male and female normal offspring rats. PS can partially inhibit effects of HFD on weight gain of PS male offspring rats, but exert no significant effects on PS female offspring rats. HFD can aggravate PS-induced depression-like behavior in offspring rats.

Abstract Background: LincRNAs have been revealed to be tightly associated with various tumorigeneses and cancer development, but the roles of specific lincRNA on tumor-related angiogenesis was hardly studied. Here, we aimed to investigate whether linc-OIP5 in breast cancer cells affects the angiogenesis of HUVECs and whether the linc-OIP5 regulations are involved in angiogenesis-related Notch and Hippo signaling pathways. Methods: A trans-well system co-cultured HUVECs with linc-OIP5 knockdown breast cancer cell MDA-MB-231 was utilized to study the proliferation, migration and tube formation abilities of HUVECs and alterations of related signaling indicators in breast cancer cells and their conditioned medium through a series of cell and molecular experiments. Results: Overexpressed linc-OIP5, YAP1, and JAG1 were found in breast cancer cell lines MCF7 and MDA-MB-231 and the expression levels of YAP1 and JAG1 were proportional to the breast cancer tissue grades. MDA-MB-231 cells with linc-OIP5 knockdown led to weakened proliferation, migration, and tube formation capacity of co-cultured HUVECs. Besides, linc-OIP5 knockdown in co-cultured MDA-MB-231 cells showed downregulated YAP1 and JAG1 expression, combined with a reduced JAG1 level in conditioned medium. Furthermore, a disrupted DLL4/Notch/NRP1 signaling in co-cultured HUVECs were also discovered under this condition. Conclusion: Hence, linc-OIP5 in MDA-MB-231 breast cancer cells may act on the upstream of the YAP1/Notch/NRP1 signaling circuit to affect proliferation, migration, and tube formation of co-cultured HUVECs in a non-cellular direct contact way through JAG1 in conditioned medium. These findings at least partially provide a new angiogenic signaling circuit in breast cancers and suggest linc-OIP5 could be considered as a therapeutic target in angiogenesis of breast cancers.

Abstract To study the influence of a foundation structure on the ground, the finite layer method of composite ground is derived based on the finite layer method (FLM) theory of the semi-analytic numerical method and the force method principle of elastic material, which is used to analyze a layered ground including multiple internal constraint planes. By simplifying a pile body as a solid constraint composed of four side plane constraints, the composite ground FLM is applied to solve the displacement and stress field of the ground. The change of the ground displacement and stress caused by the addition of a single pile constraint are then discussed, and the influence rules of a pile position on the ground displacement are also determined. Finally, the necessity, rationality and validity of the composite ground FLM are demonstrated by the model test examples of a single pile and pile raft foundation.

Objective of the article was to assess the coupling effects of zeolite (Z0, 0 t ha-1; Z0.9 and Z0.22, 10 t ha-1 in different diameter of 0.45-0.9 mm and 0.17-0.22 mm) and Nitrogen (N0, N52.5, N105, N157.5 kg ha-1) application on N use, leaf area index (LAI) and rice grain yield in 2014 and 2015. Results showed that both Z0.9 and Z0.22 at N105 and N157.5 application rates could greatly enhance the biomass, dry weight of root, LAI and N uptake. However, at initial tillering stage of rice, Z decreased N content in plant for its high absorption capacity and hence lowered biomass and N uptake. The highest value of N recovery efficiency (NRE) and N use efficiency (NUE) was obtained from N105Z0.22. Z input contributed to higher NRE and NUE. At N157.5 application rate, Z0.9 and Z0.22 significantly increased rice grain yield by 14.6% and 15.3%, respectively. However, Z mattered little to rice grain yield at N0 and N52.5. There was no significant difference in rice grain yield between Z0.22 and Z0.9. Z0.22 and Z0.9 clearly increased the 1000-grain weight. N application significantly lowered unfilled grain percentage and 1000-grain weight.

Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively, were further studied by real time RT-PCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends. Conclusions: The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed.

To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).